On MAR 27th,Bioscience,Biotechnology, and Biochemistry, a SCI Journal,pubished the research of our company online titled "Inhibitory effects of 3α-hydroxy-lup-20(29)-en-23,28-dioic acid on lipopolysaccharide-induced TNF-α, IL-1β, and the high mobility group box 1 release in macrophages"

In this work, the anti-inflammatory effects of a lupane-type triterpene isolated from leaves of Acanthopanax gracilistylus W.W.Smith, as well as the underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW264.7 cells.The results demonstrated that the lupane-type triterpene dose-dependently reduced the production of nitric oxide (NO), significantly suppressed the LPS-induced expression of TNF-α and IL-1β at the mRNA and protein levels in RAW264.7 cells. Further analysis revealed that the lupane-type triterpene could reduce the secretion of High Mobility Group Box 1 (HMGB1). Additionally, it inhibited nuclear translocation of NF-κB p65 in LPS-stimulated RAW264.7 macrophages. In conclusion, this study demonstrates that the lupane-type triterpene exerts anti-inflammatory effects in LPS-stimulated RAW 264.7 macrophages by playing a role in the inhibition of NF-κB, further studies are necessary to determine the precise mechanism of the lupane-type triterpene’s action on NF-κB. These results warrant further studies that would concerning the candidate therapy for diseases such as fulminant hepatitis and rheumatology of lupane-type triterpenoids containing in AGS.

 

The research was supported by Natural Science Foundation of Hunan Province, China under grant 11JJ2042, and Key Projects of Changsha City Science and Technology Bureau under grant k1403122-31.

 

Our company,Changsha Broad-Ocean Bio-science and Technique Co.,Ltd.is one of the primary completion institue.Our president Xiangqian Liu, and Qinpeng Zou contribute equally as first authors.